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HelpTest ID: TLPMF T-Cell Lymphoma, Specified FISH, Varies
Ordering Guidance
This test should only be ordered if the sample is known to have a sufficient clonal T-cell population.
This test is intended for instances when targeted T-cell lymphoma fluorescence in situ hybridization (FISH) probes are needed based on a specific abnormality or abnormalities identified in the diagnostic sample. If targeted FISH probes are not included with this test order, test processing may be delayed and the test may be canceled by the laboratory and automatically reordered by the laboratory as TLPDF / T-Cell Lymphoma, Diagnostic FISH, Varies.
If the entire T-cell lymphoma panel is preferred, order TLPDF / T-Cell Lymphoma, Diagnostic FISH, Varies.
This test should NOT be used to screen for residual T-cell lymphoma.
This assay detects chromosome abnormalities observed in blood or bone marrow samples of patients with T-cell lymphoma. If a paraffin-embedded tissue specimen is submitted, the test will be canceled and TLYM / T-Cell Lymphoma, FISH, Tissue will be added and performed as the appropriate test.
For patients with T-cell acute lymphoblastic leukemia/lymphoma, order either TALAF / T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), FISH, Adult, Varies or TALPF / T-Cell Acute Lymphoblastic Leukemia/Lymphoma (ALL), FISH, Pediatric, Varies, depending on the age of the patient.
For testing paraffin-embedded tissue samples from patients with T-cell lymphoblastic Lymphoma, see TLBLF / T-Lymphoblastic Leukemia/Lymphoma, FISH, Tissue.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
1. A list of probes requested for analysis is required. Probes available for this test are listed in the Testing Algorithm section.
2. A reason for testing must be provided. If this information is not provided, an appropriate indication for testing may be entered by Mayo Clinic Laboratories.
3. A flow cytometry and/or a bone marrow pathology report should be submitted with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
Specimen Required
Submit only 1 of the following specimens:
Preferred
Specimen Type: Bone marrow
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 2-3 mL
Collection Instructions:
1. It is preferable to send the first aspirate from the bone marrow collection.
2. Invert several times to mix bone marrow.
3. Send bone marrow in original tube. Do not aliquot.
Acceptable
Specimen Type: Whole Blood
Container/Tube:
Preferred: Yellow top (ACD)
Acceptable: Green top (heparin) or lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
Useful For
Detecting, at diagnosis, common chromosome abnormalities associated with specific T-cell lymphoma subtypes using client specified probes
Testing Algorithm
This test includes a charge for the probe application, analysis, and professional interpretation of results for one probe set (2 individual fluorescence in situ hybridization [FISH] probes). Additional charges will be incurred for all additional probe sets performed.
When specified, any of the following probes will be performed:
t(14q32.1;var) or TCL1A rearrangement, request probe TCL1A break-apart
t(14q11.2;var) or TRAD rearrangement, request probe TRAD break-apart
i(7q) or isochromosome 7q, request probe D7Z1/D7S486
+8 or trisomy 8, request probe D8Z2/MYC
TCL1A and TRAD break-apart probe sets will be performed simultaneously and cannot be ordered independently.
Appropriate ancillary probes may be performed at consultant discretion to render comprehensive assessment. Any additional probes will have the results included within the final report and will be performed at an additional charge.
For more information see Bone Marrow Staging for Known or Suspected Malignant Lymphoma Algorithm.
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
T-cell Lymphoma B/BM, Spec FISHSpecimen Type
VariesSpecimen Minimum Volume
Bone marrow: 1 mL; Whole blood: 2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Clinical Information
T-cell malignancies account for approximately 10% of all non-Hodgkin lymphomas. There are subtypes of T-cell lymphoma with diagnostic and prognostic genetic abnormalities. Fluorescence in situ hybridization (FISH) is available for specific abnormalities in the following T-cell lymphoma subtypes (see Table).
Table. Common Chromosome Abnormalities in T-cell Lymphomas
|
Lymphoma type |
Chromosome abnormality |
FISH probe |
|
T-cell prolymphocytic leukemia
|
inv(14)(q11q32)/ (14;14)(q11;q32) |
5'/3' TRAD |
|
Hepatosplenic T-cell lymphoma |
Isochromosome 7q |
D7Z1/ D7S486 |
|
Trisomy 8 |
D8Z2/MYC |
Reference Values
An interpretive report will be provided.
Interpretation
A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe set.
Detection of an abnormal clone supports a diagnosis of T-cell lymphoma. The specific abnormality detected may help to determine a specific T-cell lymphoma subtype.
The absence of an abnormal clone does not rule out the presence of lymphoma or another neoplastic disorder.
Clinical Reference
1. Swerdlow S, Campo E, Harris NL, et al, eds. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. IARC Press; 2017
2. Gesk S, Martin-Subero JI, Harder L, et al. Molecular cytogenetic detection of chromosomal breakpoints in T-cell receptor gene loci. Leukemia. 2003;17(4):738-745
3. Chin M, Mugishima H, Takamura M, et al. Hemophagocytic syndrome and hepatosplenic (gamma)(delta) T-cell lymphoma with isochromosome 7q and 8 trisomy. J Pediatr Hematol Oncol. 2004;26(6):375-378
Day(s) Performed
Monday through Friday
Report Available
7 to 10 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88271x2, 88275 x1, 88291x1- FISH Probe, Analysis, Interpretation; 1 probe set
88271x2, 88275 x1 - FISH Probe, Analysis; each additional probe set (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TLPMF | T-cell Lymphoma B/BM, Spec FISH | 101682-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 614348 | Result Summary | 50397-9 |
| 614349 | Interpretation | 69965-2 |
| 614350 | Result Table | 93356-4 |
| 614351 | Result | 62356-1 |
| GC141 | Reason for Referral | 42349-1 |
| GC142 | Probes Requested | 78040-3 |
| GC143 | Specimen | 31208-2 |
| 614352 | Source | 31208-2 |
| 614353 | Method | 85069-3 |
| 614354 | Additional Information | 48767-8 |
| 614355 | Disclaimer | 62364-5 |
| 614356 | Released By | 18771-6 |
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.
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