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Test ID: FIBR Fibroblast Culture


Necessary Information


1. Include patient clinical history with request. This information will be used for any testing performed on the cultured cells.

2. Indicate the tests to be performed on the fibroblast culture cells.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Cultured fibroblasts

Container/Tube: T-75 or T-25 flask

Specimen Volume: 1 Full T-75 flask or 2 full T-25 flasks

Specimen Stability Information: Ambient/ Refrigerated

 

Specimen Type: Skin biopsy

Supplies: Fibroblast Biopsy Transport Media (T115)

Container/Tube: Sterile container with any standard cell culture media (eg, minimal essential media, RPMI 1640). The solution should be supplemented with 1% penicillin and streptomycin. Tubes of culture media can be supplied upon request (Eagle's minimum essential medium with 1% penicillin and streptomycin [T115]).

Specimen Volume: 4-mm punch

Specimen Stability Information: Refrigerated/Ambient


Forms

Biochemical Genetics Patient Information (T602) in Special Instructions.

Useful For

A preliminary step in obtaining cultured cells for specialized testing including enzymatic and molecular genetic

Additional Tests

Test ID Reporting Name Available Separately Always Performed
CRYOB Cryopreserve for Biochem Studies No Yes

Testing Algorithm

When this test is ordered, cryopreservation for biochemical studies will always be performed at an additional charge. However, for multiple assays on a patient utilizing the ordered fibroblast culture, only 1 culture is required regardless of the number of assays ordered.

 

If viable cells are not obtained within 30 days, client will be notified.

 

Mycoplasma screening will be performed on all successfully grown fibroblast cultures. If Mycoplasma is detected, all pending fibroblast clinical testing will be cancelled and the samples will be discarded.

 

See Newborn Screening Follow-up for Isolated C5 Acylcarnitine Elevations (also applies to any plasma or serum C5 acylcarnitine elevations) in Special Instructions.

Method Name

FIBR: Cultivated from Biopsy as Monolayer

CRYOB: Fibroblast Subculture Followed by Cryopreservation and Storage

Reporting Name

Fibroblast Culture

Specimen Type

Tissue

Specimen Stability Information

Specimen Type Temperature Time
Tissue Varies

Clinical Information

Cultures of skin fibroblasts are useful for specialized testing, which requires skin cells. These cells can be cultured and tested at Mayo Clinic or sent to external laboratories performing these specialized tests. In addition, cells are frozen for at least 3 years for potential future studies on cultured cells or for molecular genetic testing.

Reference Values

Not applicable

Day(s) and Time(s) Performed

Varies

Analytic Time

40-45 days depending on rapidity of growth

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88233-Fibroblast culture

88240-Cryopreservation for biochemical studies

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FIBR Fibroblast Culture In Process

 

Result ID Test Result Name Result LOINC Value
8482 Fibroblast Culture In Process
Mayo Clinic Laboratories | Genetics and Pharmacogenomics Catalog Additional Information:

mml-biochemical