Home
HelpTest ID: DICET DICER1 Mutation Analysis, Next-Generation Sequencing, Tumor
Ordering Guidance
Multiple oncology (cancer) gene panels are available. For more information see Hematology, Oncology, and Hereditary Test Selection Guide.
Necessary Information
A pathology report (final or preliminary), at minimum containing the following information, must accompany specimen for testing to be performed:
1. Patient name
2. Block number-must be on all blocks, slides, and paperwork (can be handwritten on the paperwork)
3. Tissue collection date
4. Source of the tissue
Specimen Required
This assay requires at least 20% tumor nuclei.
-Preferred amount of tumor area with sufficient percent tumor nuclei: tissue 216 mm(2)
-Minimum amount of tumor area: tissue 36 mm(2)
-These amounts are cumulative over up to 10 unstained slides and must have adequate percent tumor nuclei.
-Tissue fixation: 10% neutral buffered formalin, not decalcified
-For specimen preparation guidance, see Tissue Requirement for Solid Tumor Next-Generation Sequencing. In this document, the sizes are given as 4 mm x 4 mm x 10 slides as preferred: approximate/equivalent to 144 mm(2) and the minimum as 3 mm x 1 mm x 10 slides: approximate/equivalent to 36 mm(2).
Preferred:
Specimen Type: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block with acceptable amount of tumor tissue.
Acceptable:
Specimen Type: Tissue slide
Slides: 1 Stained and 10 unstained
Collection Instructions: Submit 1 slide stained with hematoxylin and eosin and 10 unstained, nonbaked slides with 5-micron thick sections of the tumor tissue.
Note: The total amount of required tumor nuclei can be obtained by scraping up to 10 slides from the same block.
Additional Information: Unused unstained slides will not be returned.
Specimen Type: Cytology slide (direct smears or ThinPrep)
Slides: 1 to 3 Slides
Collection Instructions: Submit 1 to 3 slides stained and coverslipped with a preferred total of 5000 nucleated cells, or a minimum of at least 3000 nucleated cells.
Note: Glass coverslips are preferred; plastic coverslips are acceptable but will result in longer turnaround times.
Additional Information: Cytology slides will not be returned.
Useful For
Identifying specific mutations within the DICER1 gene to assist in tumor diagnosis/classification
Genetics Test Information
This test uses targeted next-generation sequencing to evaluate for somatic mutations within the DICER1 gene. See Targeted Genes and Methodology Details for DICER1 Mutation Analysis for details regarding the targeted gene regions evaluated by this test.
This test is performed to evaluate for somatic mutations within solid tumor samples. This test does not assess for germline alterations within the DICER1 gene.
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| SLIRV | Slide Review in MG | No, (Bill Only) | Yes |
Testing Algorithm
When this test is ordered, slide review will always be performed at an additional charge.
Method Name
Sequence Capture Next-Generation Sequencing (NGS)
Reporting Name
DICER1 Mutation Analysis, TumorSpecimen Type
VariesSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Clinical Information
This test uses formalin-fixed paraffin-embedded tissue or cytology slides to assess for somatic mutations involving the DICER1 gene known to be associated with multiple tumor types. DICER1 mutations are a diagnostic marker of embryonal rhabdomyosarcoma and Sertoli-Leydig cell tumors.(1) In central nervous system (CNS) tumors, DICER1 mutations are a diagnostic molecular biomarker for primary intracranial sarcoma, DICER1-mutant, embryonal tumor with multilayered rosettes and pituitary blastoma.(2) DICER1 mutations also occur in a subset of pineoblastoma, typically in the context of DICER1 tumor-predisposition syndrome. DICER1 tumor-predisposition syndrome is an inherited predisposition to pleuropulmonary blastoma, cystic nephroma, Wilms' Tumor, anaplastic sarcoma of the kidney, ovarian Sertoli-Leydig cell tumor, and cervical embryonal rhabdomyosarcoma.
Reference Values
An interpretive report will be provided.
Interpretation
The interpretation of molecular biomarker analysis includes an overview of the results and the associated diagnostic, prognostic, and therapeutic implications.
Clinical Reference
1. WHO Classification of Tumours Editorial Board. Female genital tumours. 5th ed. World Health Organization; 2020. WHO Classification of Tumours. Vol 4
2. WHO Classification of Tumours Editorial Board: Central nervous system tumours. 5th ed. World Health Organization; 2021. WHO Classification of Tumours. Vol 6
3. Strom SP. Current practices and guidelines for clinical next-generation sequencing oncology testing. Cancer Biol Med. 2016;13(1):3-11. doi:10.28092/j.issn.2095-3941.2016.0004
4. Spurr L, Li M, Alomran N, et al. Systematic pan-cancer analysis of somatic allele frequency. Sci Rep. 2018;8(1):7735. Published 2018 May 16. doi:10.1038/s41598-018-25462-0
5. Caroleo AM, De loris MA, Boccuto L, et al: DICER1 syndrome and cancer predisposition: From a rare pediatric tumor to lifetime risk. Front Oncol. 2021 Jan 21;10:614541
6. Li BK, Vasiljevic A, Dufour C, et al: Pineoblastoma segregates into molecular sub-groups with distinct clinico-pathologic features: a rare brain tumor consortium registry study. Acta Neuropathol. 2020 Feb;139(2):223-241
7. Koelsche C, Mynarek M, Schrimpf D, et al: Primary intracranial spindle cell sarcoma with rhabdomyosarcoma-like features share a highly distinct methylation profile and DICER1 mutations. Acta Neuropathol. 2018 Aug;136(2):327-337
8. de Kock L, Yoon JY, Apellaniz-Ruiz M, et al: Significantly greater prevalence of DICER1 alterations in uterine embryonal rhabdomyosarcoma compared to adenosarcoma. Mod Pathol. 2020 Jun;33:1207-1219
9. McCluggage WG, Apellaniz-Ruiz M, Chong AL, et al: Embryonal rhabdomyosarcoma of the ovary and fallopian tube: Rare neoplasms associated with germline and somatic DICER1 mutations. Am J Surg Pathol. 2020 Jun;44:738-747
10. de Kock L, Terzic T, McCluggage WG, et al: DICER1 mutations are consistently present in moderately and poorly differentiated sertoli-leydig cell tumors. Am J Surg Pathol. 2017 Sep;41:1178-1187
Day(s) Performed
Monday through Friday
Report Available
12 to 20 daysTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88381-Microdissection, manual
81479
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DICET | DICER1 Mutation Analysis, Tumor | 105585-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 619668 | Result | 82939-0 |
| 619669 | Interpretation | 69047-9 |
| 619670 | Additional Information | 48767-8 |
| 619671 | Specimen | 31208-2 |
| 619672 | Tissue ID | 80398-1 |
| 619673 | Method | 48767-8 |
| 619674 | Disclaimer | 62364-5 |
| 619675 | Released By | 18771-6 |
Forms
If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.
mcl-moltechtestmenu; mcl-oncology