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HelpTest ID: CULFB Fibroblast Culture for Molecular Testing, Chorionic Villi/Products of Conception
Useful For
Producing cell cultures that can be used for genetic analysis
Special Instructions
Method Name
Cell Culture
Reporting Name
Fibroblast Culture for Genetic TestSpecimen Type
TissueOrdering Guidance
This test is most useful for chorionic villus sampling and products of conception specimens.
This test is not appropriate for culturing other tissue specimens such as skin biopsies. If this test is ordered on other tissue types, the test will be canceled and FIBR/ Fibroblast Culture, Tissue will be performed as the appropriate test.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service
Necessary Information
Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
The additional tests desired must be indicated on the request form that accompanies the specimen.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Autopsy
Supplies: Hank's Solution (T132)
Container/Tube: Sterile container with sterile Hank's balanced salt solution, Ringer's solution, or normal saline
Specimen Volume: 4-mm diameter
Collection Instructions:
1. Wash biopsy site with an antiseptic soap.
2. Thoroughly rinse area with sterile water.
3. Do not use alcohol or iodine preparations.
4. Biopsy specimens are best taken by punch biopsy to include full thickness of dermis.
Specimen Type: Chorionic villi
Supplies: CVS Media (RPMI) and Small Dish (T095)
Container/Tube: 15 mL tube containing 15 mL of transport media
Specimen Volume: 20-30 mg
Collection Instructions:
1. Collect specimen by the transabdominal or transcervical method.
2. Transfer the chorionic villi specimen to a Petri dish containing transport medium.
3. Using a stereomicroscope and sterile forceps, assess the quality and quantity of the villi and remove any blood clots and maternal decidua.
Specimen Type: Products of conception or stillbirth
Supplies: Hank's Solution (T132)
Container/Tube: Sterile container with sterile Hank's balanced salt solution, Ringer's solution, or normal saline
Specimen Volume: 1 cm(3) of placenta (including 20 mg of chorionic villi) and a 1 cm(3) biopsy specimen of muscle/fascia from the thigh
Collection Instructions: If a fetus cannot be specifically identified, collect 50 mg villus material or tissue that appears to be of fetal origin.
Additional Information: Do not send entire fetus.
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Tissue | Refrigerated (preferred) | ||
| Ambient | |||
Clinical Information
Cultured cells may be used to perform a wide range of laboratory tests. Prior to testing, the tissue may need to be cultured to obtain adequate numbers of cells.
Day(s) Performed
Monday through Friday
Report Available
41 to 42 daysTest Classification
Not ApplicableCPT Code Information
88233
88240
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CULFB | Fibroblast Culture for Genetic Test | 96300-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 52327 | Result Summary | 50397-9 |
| 52329 | Interpretation | 69965-2 |
| 52328 | Result | 82939-0 |
| CG770 | Reason for Referral | 42349-1 |
| CG899 | Specimen | 31208-2 |
| 52331 | Source | 31208-2 |
| 52332 | Method | 85069-3 |
| 54625 | Additional Information | 48767-8 |
| 52333 | Released By | 18771-6 |
Forms
Final Disposition of Fetal/Stillborn Remains (if fetal specimen is sent) in Special Instructions. Only required for products of conception or stillbirth specimen.
Testing Algorithm
This processing test is for culturing chorionic villus or products of conception specimens for cytogenetic, molecular genetic, or other testing. No analysis or interpretation of results is performed.
Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested.
Specimen Minimum Volume
See Specimen Required
Reference Values
Not applicable
Clinical Reference
Arsham MS, Barch MJ, Lawce HJ, eds: The AGT Cytogenetics Laboratory Manual. 4th ed. John Wiley and Sons Inc; 2017
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