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Test ID: CULAF Culture for Genetic Testing, Amniotic Fluid

Useful For

Producing amniocyte cultures that can be used for genetic analysis

Method Name

Cell Culture

Reporting Name

Amniotic Fluid Culture/Genetic Test

Specimen Type

Amniotic Fld


Shipping Instructions


Advise Express Mail or equivalent if not on courier service.



Necessary Information


Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

 

The additional tests desired must be indicated on the request form that accompanies the specimen.



Specimen Required


Optimal timing for specimen collection is during 14 to 18 weeks of gestation, but specimens collected at other weeks of gestation are also accepted.

 

Container/Tube: Amniotic fluid container

Supplies: Refrigerate/Ambient Shipping Box, 5 lb (T329)

Specimen Volume: 5 to 10 mL

Collection Instructions:

1. Discard the first 2 mL of amniotic fluid. If the culture will be performed in conjunction with other cytogenetic testing such as CHRAF / Chromosome Analysis, Amniotic Fluid or CMAP / Chromosomal Microarray, Prenatal, Amniotic Fluid/Chorionic Villus Sampling, a total of 25 to 30 mL will be needed.

2. Place the tubes in a Styrofoam container.

3. Fill remaining space with packing material.

4. Unavoidably, about 1% to 2% of mailed-in specimens are not viable.

5. Bloody specimens are undesirable.


Specimen Minimum Volume

5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Amniotic Fld Refrigerated (preferred)
  Ambient 

Clinical Information

Fetal cells obtained by amniocentesis (amniocytes) are used for a wide range of laboratory tests. Prior to testing, the cells may need to be cultured to obtain adequate numbers of amniocytes.

Reference Values

Not applicable

Clinical Reference

The AGT Cytogenetics Laboratory Manual Fourth edition. Edited by MS Arsham, MJ Barch, HJ Lawce. John Wiley and Sons Inc, Hoboken, NJ, 2017

Day(s) Performed

Monday through Friday

Report Available

23 to 24 days

Test Classification

Not Applicable

CPT Code Information

88235

88240

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CULAF Amniotic Fluid Culture/Genetic Test In Process

 

Result ID Test Result Name Result LOINC Value
52304 Result Summary 50397-9
52306 Interpretation 69965-2
52305 Result 82939-0
CG767 Reason for Referral 42349-1
52307 Specimen 31208-2
52308 Source 31208-2
52309 Method 85069-3
54641 Additional Information 48767-8
52310 Released By 18771-6

Testing Algorithm

This processing test is for culturing amniocytes for prenatal genetic testing.

 

Once confluent flasks are established, the amniocyte cultures are sent to other laboratories, either within Mayo Clinic or to external sites, based on the specific testing requested.

Mayo Clinic Laboratories | Genetics and Pharmacogenomics Catalog Additional Information:

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