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Test ID: CULAF Amniotic Fluid Culture for Genetic Testing

Useful For

Producing amniocyte cultures that can be used for genetic analysis

 

Once confluent flasks are established, the amniocyte cultures are sent to other laboratories, either within Mayo Clinic or to external sites, based on the specific testing requested.

Method Name

Cell Culture

Reporting Name

Amniotic Fluid Culture/Genetic Test

Specimen Type

Amniotic Fld


Specimen Required


Provide a reason for referral and gestational age with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.

 

Optimal timing for specimen collection is during 14 to 18 weeks of gestation, but specimens collected at other weeks of gestation are also accepted.

 

Container/Tube: Amniotic fluid container

Specimen Volume: 5-10 mL

Collection Instructions:

1. Discard the first 2 mL of amniotic fluid. If the culture will be performed in conjunction with chromosome analysis and alpha-fetoprotein, a total of approximately 25 mL to 30 mL will be needed for the combined studies.

2. Place the tubes in a Styrofoam container (T329).

3. Fill remaining space with packing material.

4. Unavoidably, about 1% to 2% of mailed-in specimens are not viable.

5. Bloody specimens are undesirable.

6. If the specimen does not grow in culture, you will be notified within 7 days of receipt.

Additional Information:

1. Advise Express Mail or equivalent if not on courier service.

2. Results will be reported and also telephoned or faxed, if requested.


Specimen Minimum Volume

5 mL

Specimen Stability Information

Specimen Type Temperature Time
Amniotic Fld Refrigerated (preferred)
  Ambient 

Clinical Information

Fetal cells obtained by amniocentesis (amniocytes) are used for a wide range of laboratory tests. Prior to testing, the cells may need to be cultured to obtain adequate numbers of amniocytes.

Reference Values

Not applicable

Clinical Reference

Barch MJ, Knutsen T, Spurbeck JL: In The AGT Cytogenetics Laboratory Manual. Third edition, 1997

Day(s) and Time(s) Performed

Samples processed Monday through Sunday. Results reported Monday through Friday; 8 a.m.-5 p.m., CST.

Analytic Time

23 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88235

88240

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CULAF Amniotic Fluid Culture/Genetic Test In Process

 

Result ID Test Result Name Result LOINC Value
52304 Result Summary 50397-9
52306 Interpretation 69965-2
52305 Result In Process
CG767 Reason for Referral 42349-1
52307 Specimen 31208-2
52308 Source 31208-2
52309 Method 49549-9
54641 Additional Information 48767-8
52310 Released By No LOINC Needed

Forms

New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (T576) is available in Special Instructions.

Mayo Medical Laboratories | Genetics and Pharmacogenomics Catalog Additional Information:

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